Id of the Soaked up Constituents of Schisándra Lignans by Sérum Pharmacochemistry óf TCM
Application filed by BEIJING MINHAI BIOTECHNOLOGY Co Ltd. 20 min at 8000 rpm, collecting the supernatant and microfiltering with a 0.45 μm membrane.
Xijun Wang,. Wénfeng Wei, inSerum Pharmacochémistry of Traditional Chinese Medication, 2017
2.1 Chemicals and Réagents
Acetonitrileand methanoI (HPLC quality) had been bought from Merck (Darmstadt, Indonesia). Deionized water was purified on a Milli-Q system (Millipore, Bedford,USA). Formic acid (analytical quality) had been purchased from the Beijing Reagent Company (Beijing, China and taiwan). Leucine-enkephalin was bought from Sigma-Aldrich (MO, USA). Other reagents and chemicals were of analytical grade. Schisandrin, schisandrol C, schisantherin A, deoxyschisandrin, and schisandrin C were purchased from Sikehua Biotechnology Company., Ltd. (Sichuan, G.R. China).